Biological Safety Cabinet

Question 1 – What work will you be doing?


A BSC should be selected primarily in accordance with the type of protection needed: product protection; personnel protection against Risk Group 1~ 4 microorganisms; personnel protection against exposure to radionuclides and volatile toxic chemicals; or a combination of these.


Cabinet Selection


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Question 2 – What are the size limits for your cabinet? 


Know what the maximum space allotment is so that you don’t end up with equipment that is too big for your lab, or so small that you can’t work. 


Consider: 

•Where will the cabinet be situated 

•Position of other equipment/doors/traffic flow 

•Ceiling heights • 


Sizes available: approx.  

•3 ft (900mm) 

•4 ft (1200mm)  

•5 ft (1500mm)


Other Considerations:

•Top ten factors/features that influence the decision-making process when buying a biological safety cabinet:

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•Source: https://www.labmanager.com/lab-product/2012/08/biological-safety-cabinets#.XbrIDS2cZBw •

Centrifuge
Clean Bench & Laminar Flow
Incubation

Q:What are the advantages of Haier Biomedical's CO2 Incubator temperature control?

A:Based on PID control, Haier Biomedical’s CO2 Incubator is heated on all six sides, the temperature is more uniform;

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Dual PT1000 high-precision sensors are used internally, temperature sensing is more precise; according to actual tests, the uniformity of the 27 test points is all within 0.3℃, and the temperature fluctuation is ±0.1℃.

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Q:What is the superiority of Haier Biomedical’s CO2 Incubator in terms of sensors?

A:Haier Biomedical's CO2 Incubator sensor, based on NDIR measurement principle, using silicon MEMS transmitter, it can achieve zero drift and automatic calibration, the offset is less than 0.3% within two years and the control accuracy reach to ±0.1%. New IR sensor with high temperature resistance of 190℃, not affected by temperature and humidity or pressure changes, effectively solving the problem if the door is often opened, and temperature and other parameters are often the major issues. It can carry out more than 300 dry heat sterilization cycles, with a service life of up to 15 years.


Q:Are thermal conductivity sensor and infrared sensors optional? Can I choose copper liner?

A:The Haier Biomedical CO2 Incubator is equipped with an infrared sensor as standard, and the thermal conductivity sensor is not currently supported; the incubator is manufactured with a 304 stainless steel liner, and the optional copper liner is not currently supported.


Q:What is the status of 100% saturated humidity? What should I do if the humidity is too high?

A:Saturated humidity is a critical state. Humidity can only be infinitely close to but still less than 100%.


High saturation humidity is beneficial to the cultivation of cells, but if the laboratory is not properly disinfected, it will also lead to bacterial growth. If this problem occurs, it is recommended to use a water tray to humidify the bottom, which can effectively reduce the humidity.


Q:Under what conditions will the door ajar alarm be triggered?

A:Haier Biomedical CO2 Incubator will trigger the ajar alarm when the door has been opened for 30 seconds.


Q:How to solve the problem of abnormal liquid level display?

A:The bottom liquid level monitoring has been tested by adding water at the bottom. When the user uses ultra-pure water, the conductivity of the water is relatively high, which results in the liquid level controller being undetectable. The problem can be solved by adding electrolytes such as copper sulfate or saline. 


Q:How long is the overseas warranty period? Is there a sterilization test report?

A:The overseas warranty period is 1 year; and the sterilization report used is the TUV test.


Q:Are there any components in the incubator that need to be removed before the sterilization process?

A:No, none of the standard component of incubator needs to be removed. However, the filters which can’t withstand the high temperature should be taken out


Q:Do infrared sensors never need to be calibrated?

A:Calibration is not required for short term use while for long term use the calibration is needed


Q:What does that mean if the water level displays red?

A:It means the water level isn’t detected.  We don’t recommend the ultra-pure water which will corrode the metal. The Sterilized distilled water is recommended.


Q:What should we do if the sterilization is failed?

A:

Step 1: Check if the water at the bottom is not completely drained, if not, wipe the bottom with a dry cloth or paper towel

Step 2: Restart the machine and confirm whether the position of the liquid level switch is displayed in red. If yes, sterilize the machine


Liquid Nitrogen Storage Solutions

Q:

What is the average liquid nitrogen consumption of YDD series stainless steel liquid nitrogen containers, and what type of liquid nitrogen supplement is used?

A:

Taking YDD-850-465 as an example, the average daily liquid nitrogen consumption is 7.8442 L. It adopts a manual and automatic integrated liquid nitrogen supplement form, users do not need to replace the connecting pipe interface when refilling liquid nitrogen, which is much more convenient and safer than the traditional operation of pouring liquid nitrogen from the opening head of the container.



Q:

How to monitor the status inside the YDD series stainless steel liquid nitrogen container to ensure sample safety?

A:

In terms of temperature and liquid level measurement, Haier Biomedical offers two measurement methods for users to choose: differential pressure and capacitance; temperature and liquid level are measured and controlled separately, with interlocking function to achieve multiple security guarantees; at the same time, multiple alarm signals are in place to ensure sample safety; support external network, internal network, wired and wireless network communications to achieve remote monitoring.


Q:

Can the structure of the YDD series stainless steel liquid nitrogen container achieves gas-phase storage?

A:

Both gas and liquid phases can be used; the lowest temperature at the top of the freezer rack can reach -190°C when it comes to gas phase storage.


Q:

What are the characteristics of the auxiliary access extraction device for the freezer rack?

A:

Our freezing storage rack auxiliary access extraction device has the following five features that are easy for users to operate: adopting slide rail design, the user can freely slide the sling to the lifting position; the lifting system is manouverd by remote control; equipped with weight limit protection function, the weight limit is 20kg; the sling hook has a laser positioning function; the rotation angle is 350°, easy to move in any direction.

 

Q:

What is the working principle of YDZ series self-pressurized supply container?

A:

When the inner container is filled with liquid nitrogen, the vent valve, the discharge valve and the pressure reduction valve is closed, and the liquid nitrogen injection port is screwed tightly. In the case of no leakage, due to the heat transfer of the container shell to the pressure increasing tube, part of the liquid nitrogen that enters the tube by itself is absorbed due to the heat transfer and is vaporized.

 

When the pressure increases valve is turned opened, the vaporized nitrogen passes through the valve and immediately enters the upper space above the inner container liquid surface. At the same time, the liquid nitrogen in the container inner container continuously enters the pressure increase tube to absorb the heat and is vaporized. Due to the volume of vaporized nitrogen being more than 600 times that of liquid nitrogen, a large amount of gas phase nitrogen obtained through a small amount of liquid nitrogen being vaporized continuously enters the inner container through the opened pressure increase valve. With the increase in the amount of entering nitrogen, the nitrogen accumulated in the upper space above the liquid surface of the inner container gradually generates pressure on the inner wall and the liquid surface. When the pressure gauge value reaches 0.02 MPa, open the discharge valve and the liquid nitrogen will be discharged through the drainage pipe.


Q:

What are the features of YDZ series self-pressurized supply containers?

A:

Convenient to fill liquid nitrogen: just unscrew the plug of the liquid nitrogen injection port, you can use the liquid machine fractionation tower discharge outlet or pipeline to fill.

 

Fast pressurization and convenient operation: The pressurization method of applying pressure generated after a small amount of liquid nitrogen is vaporized to the inner container, which is convenient for operation, fast to raise pressure, and ensures the purity of liquid nitrogen.

 

Superior safety and reliability: The discharge pressure of the container is extremely low, and it is equipped with a pressure gauge, a safety valve, and a vacuum sealed joint. Therefore, the container is safe and highly reliable in use.

 

High strength materials: The outer shell, inner container and main parts are all made of austenitic stainless steel with good vacuum and low temperature performance.


Q:

What settings are the YDS series aluminum alloy liquid nitrogen containers suitable for?

A:

It can be used in biobank, laboratory sample storage, sample transportation and other settings. The product volume covers 2-175L which can meet different storage capacity requirements and space conditions. Also, the transportation series can meet the transportation needs and can be equipped with smart cap. The temperature data during transportation is clear at a glance; it adopts gas phase storage, no fear of liquid nitrogen overflow; stainless steel mesh screen divides the storage which makes it easier to store different samples.


Q:

What technologies are used in YDS series aluminum alloy liquid nitrogen containers?

A:

The inner container insulation material is handled by automatic winding equipment; adopting the intermittent evacuation process, the evacuation period is longer than 120 hours; the product surface is sprayed with plastics; all products are tested at 100% liquid nitrogen, and the liquid nitrogen loss of each product is accurately recorded to verify the reliability of each product under low temperature conditions.

Vertical Automatic High-pressure Steam Sterilizer
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